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    Widal Test (Slide Method)37830021% off
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    What is a Widal Test (Slide Method)?

    The Widal test is a blood test used to detect antibody titres when typhoid or paratyphoid fever (collectively called enteric fever) is suspected. Antibody titres measure certain antibodies in the blood in reaction to antigens, or foreign substances, and show how the body is responding immunologically. The Widal test specifically identifies antibodies against the O and H antigens of Salmonella Typhi and the H antigens of Salmonella Paratyphi . Introduced by F. Widal in 1896, this test employs a suspension medium with killed Salmonella Typhi bacteria as the antigen to diagnose typhoid fever in patients with fever. Therefore, individuals showing symptoms of enteric fever may benefit from a Widal test.

    Given its importance in diagnosing enteric fever, the Widal test is a vital tool for timely detection and treatment. If you are in Mumbai and experiencing enteric fever symptoms, consider taking a Widal test in Mumbai today.

    Widal Test (Slide Method) Parameters

    The Widal test is an agglutination test that detects cell clumping caused by specific antibodies (agglutinins) in the blood reacting to certain antigens. These antibodies target two key antigens of Salmonella Typhi : the O antigen and the H antigen. The test also includes the H antigens of Salmonella Paratyphi . Detecting these antibodies helps diagnose enteric fever by showing the body's immune response to these bacterial infections.

    The Widal test has four parameters that represent antibody titre levels against the bacteria responsible for enteric fever. These parameters include:

    • Salmonella Typhi O (TO)

    • Salmonella Typhi H (TH)

    • Salmonella Paratyphi A, H (AH)

    • Salmonella Paratyphi B, H (BH)

    Widal Test (Slide Method) Preparation

    Before booking the Widal test in Mumbai, consider the following factors:

    Prerequisites

    Fasting is not recommended for the Widal test.

    Best Time to Get Tested

    The Widal test can be taken at any time during the day.

    Who Should Get a Widal Test

    Individuals experiencing fever, headache, gastrointestinal issues (such as constipation, abdominal pain, or diarrhoea), and fatigue, especially if they have been in high-risk areas for typhoid or paratyphoid fever, should consider getting a Widal test.

    Cautions Before Taking the Widal Test

    For this test, there are no particular safety measures needed.

    Why Take a Widal Test (Slide Method)?

    Typhoid bacteria usually have an incubation period of 6 to 14 days, though it can extend up to 30 days. This duration is the time between being infected with the bacteria and the start of symptoms. The incubation period for paratyphoid bacteria ranges from 1 to 10 days. Most of the time, symptoms get worse over the course of 3 to 4 days.

    A doctor may request a Widal test if you exhibit symptoms of typhoid fever. Paratyphoid fever presents similar, but less severe, symptoms. These signs and symptoms include:

    • High fever or chills

    • Extreme fatigue

    • Feeling unwell, weak, or sick

    • Headache

    • Red rash on the chest and abdomen

    • Pain in the abdomen

    • Sweating

    • Constipation

    • Diarrhoea

    • Blood in the stool

    • Weight loss

    • Sore throat and persistent cough

    • Bleeding from the nose

    • Decreased heart rate

    • Loss of appetite

    • Confusion or hallucinations

    • Difficulty in concentrating

    • Poisoning of blood

    • Enlarged spleen or liver

    Keep in mind that even those vaccinated against typhoid are not completely immune, as 20–50% of vaccinated individuals may still contract the disease if exposed.

    A Widal test may be recommended for individuals at increased risk of typhoid, including:

    • Young children, as they are more vulnerable than adults to certain illnesses.

    • People who have come into proximity with an infected individual.

    • Travellers to regions where typhoid and paratyphoid are common (such as India, Bangladesh, the Middle East, and Africa).

    Widal Test (Slide Method) Results & Interpretation

    Below is a breakdown of the results of the Widal test:

    TypeAntibodyTitreInterpretation
    Salmonella TyphiTO<1:80Negative
    Salmonella TyphiTH<1:80Negative
    Salmonella ParatyphiAH<1:80Negative
    Salmonella ParatyphiBH<1:80Negative

    Interpretation

    A titre below 1:80 is deemed negative, indicating no infection in the patient.

    Titres of 1:80 or higher are generally considered positive, though this threshold can differ across populations and regions. A rising titre, seen through two or more samples taken at 4- to 6-day intervals after disease onset, holds more significance than a single high titre. Test results should be interpreted in conjunction with the patient’s medical history to confirm a diagnosis.

    Limitations

    Patients vaccinated against typhoid may exhibit elevated antibody titres, potentially resulting in false-positive outcomes. Similarly, individuals with previous infections or immunisations may also have misleading results.

    Agglutinins typically appear in the blood by the end of the first week of infection, so samples taken earlier might return negative results.

    While the serological findings of this test are important, they should not replace culture-based methods for identifying the causative organisms. Efforts should be made to recover and identify the pathogens through culture and biochemical tests.

    FAQs on Widal Test (Slide Method) in Mumbai

    The Widal price is ₹ 300. This price is inclusive of the fastest home sample collection in 60 minutes of booking, and the reports are available in 6 hours.

    A Widal test, or blood test for typhoid, detects antibodies in the blood against specific antigens of Salmonella bacteria, especially Salmonella Typhi and Salmonella Paratyphi . This test helps diagnose enteric fever, including typhoid and paratyphoid fever, by indicating the body's immune response to these bacterial infections.

    No, fasting is not needed for the Widal test or Typhoid Test at home.

    Yes, with Orange Health Labs, you can arrange home sample collection for the Widal Blood test or Typhoid Blood test, with the convenience of samples being collected within 60 minutes of booking, based on available slots.

    To book a blood test or health checkup on our platform, do the following:

    Find the Test : Look for the Widal test in Mumbai or the Typhoid test in Mumbai and click on Orange Health’s listing.

    Review and Book : Select the test, review the prerequisites, enter your address, and confirm your booking after selecting a convenient time slot for sample collection.

    Sample Collection : Our professional eMedic will come within your chosen time slot to collect the sample at your home.

    Lab Processing : The sample will be sent to our NABL accredited and ICMR approved labs for testing.

    Receive Results : You will get the reports of your Widal test at home via email or WhatsApp within 6 hours. The reports will also be available on our app.

    The Widal test does not have a universally accepted 'normal' range. Instead, titres below 1:80 are generally seen as negative, whereas titres of 1:80 or above may indicate infection. However, interpretation hinges on clinical considerations and the prevalence of the disease in the area.

    Following sample collection, you can expect to receive your results via WhatsApp and the app within 6 hours if you're located in Mumbai. Please note that turnaround times may differ in other cities.

    The frequency of Widal testing is determined by your doctor's recommendation. If you have been diagnosed with typhoid or paratyphoid fever, your doctor might suggest undergoing this test every 2 weeks, based on your individual clinical condition and symptoms.

    Parameters analysed in the Widal test involve antibody levels against particular antigens of Salmonella bacteria, such as the O and H antigens of Salmonella Typhi , as well as the H antigens of Salmonella Paratyphi .

    The Widal test detects antibodies against Salmonella bacteria, assisting in diagnosing both typhoid and paratyphoid fever.

    In contrast to the Tube technique, which mixes blood and antigen in test tubes, the Slide method mixes them on a slide.

    A blood test poses minimal risk. You might experience brief discomfort at the needle insertion site during sample collection, but it's temporary.

    A false-negative result in the Widal test incorrectly indicates the absence of antibodies despite their presence, which can occur if the test is conducted too soon after infection. Conversely, a false-positive result incorrectly indicates the presence of antibodies, possibly due to prior infections or vaccinations.

    Without having to visit a physical lab, Orange Health provides the fastest Widal test services near you across Mumbai at the convenience of your home. Localities include but are not limited to Colaba, Nariman Point, Marine Drive, Malabar Hill, Churchgate, Worli, Dadar, Parel, Byculla, Bandra, Andheri, Juhu, Ghatkopar, Powai, Chembur, Vashi, Belapur, Santacruz, Khar, Versova, Lokhandwala, Goregaon, Borivali, Kandivali, Mulund, Bhandup, Kurla.

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