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The Widal test, introduced by F. Widal in 1896, is a blood test that measures antibody titres to diagnose suspected cases of typhoid or paratyphoid fever, collectively known as enteric fever. Through the measurement of certain antibodies in the blood in reaction to antigens (foreign substances), antibody titres provide insight into the immune system's response to the body. The test detects antibodies against the O and H antigens of Salmonella Typhi and the H antigens of Salmonella Paratyphi . It uses a suspension containing killed Salmonella Typhi bacteria as the antigen to identify typhoid fever in febrile patients. Hence, those exhibiting enteric fever symptoms may find the Widal test beneficial.
Given its role in diagnosing enteric fever, the Widal test can be a critical diagnostic tool aiding in timely detection and treatment. Therefore, consider getting a Widal test in Hyderabad today, if you live in Hyderabad and are experiencing symptoms of enteric fever.
As an agglutination test, the Widal test detects the clumping of cells triggered when specific antibodies (agglutinins) in the blood react to particular antigens. These antibodies target two main antigens of Salmonella Typhi : the O antigen and the H antigen. Additionally, it includes the H antigens of Salmonella Paratyphi . The detection of these antibodies helps diagnose enteric fever by indicating the body's immune response to these bacterial infections.
The Widal test has four parameters representing the antibody titre levels against the bacteria causing enteric fever. The parameters include:
Salmonella Typhi O (TO)
Salmonella Typhi H (TH)
Salmonella Paratyphi A, H (AH)
Salmonella Paratyphi B, H (BH)
Consider the following factors, before booking the Widal test in Hyderabad:
Prerequisites
You do not need to fast before a Widal test.
Best Time to Get Tested
The Widal test can be conducted at any time during the day.
Who Should Get a Widal Test
Anyone with symptoms like gastrointestinal issues (diarrhoea, abdominal pain, or constipation), fever, fatigue, and headache, particularly if they have been in regions with a substantial risk of typhoid or paratyphoid fever, should get a Widal test.
Cautions Before Taking the Widal Test
No special precautions are needed for this test.
The incubation period for typhoid bacteria is typically 6 to 14 days, but it can be as long as 30 days. This period marks the time between infection and the onset of symptoms. The incubation time for paratyphoid bacteria might last anywhere from 1 to 10 days. Usually, symptoms get worse over the course of 3 to 4 days.
If you show symptoms of typhoid fever, a doctor might order a Widal test. Paratyphoid fever has similar but milder, symptoms. These signs and symptoms include:
High fever or chills
Sore throat and persistent cough
Headache
Extreme fatigue
Pain in the abdomen
Feeling unwell, weak, or sick
Decreased heart rate
Loss of appetite
Constipation
Diarrhoea
Sweating
Blood in the stool
Bleeding from the nose
Weight loss
Red rash on the chest and abdomen
Confusion or hallucinations
Difficulty in concentrating
Poisoning of blood
Enlarged spleen or liver
Please note: Vaccination against typhoid does not guarantee full immunity, as 20–50% of vaccinated individuals may still get the disease upon exposure.
Individuals at higher risk for typhoid might be advised to take a Widal test. These include:
Travellers to areas where typhoid and paratyphoid are prevalent, including India, Bangladesh, the Middle East, and Africa.
Those in close contact with an infected person.
Young children, who are more vulnerable to these diseases than adults.
Below is a breakdown of the results of the Widal test:
Type | Antibody | Titre | Interpretation |
---|---|---|---|
Salmonella Typhi | TO | <1:80 | Negative |
Salmonella Typhi | TH | <1:80 | Negative |
Salmonella Paratyphi | AH | <1:80 | Negative |
Salmonella Paratyphi | BH | <1:80 | Negative |
Interpretation
Titres lower than 1:80 are considered negative, suggesting the absence of infection.
Titres of 1:80 or above are typically seen as positive, but this cutoff may vary among different populations and regions. An increasing titre, observed from multiple samples taken 4 to 6 days apart after the disease begins, is more meaningful than a single elevated titre. The test results should be assessed alongside the patient's clinical findings to determine a diagnosis.
Limitations
Vaccinated patients may show elevated antibody titres, leading to possible false-positive results. Likewise, individuals with prior infections or vaccinations might also have misleading results.
Agglutinins generally appear in the blood by the end of the first week of infection, so blood samples taken earlier may result in negative findings.
Although the serological results of this test are valuable, they should not replace culture-based methods for identifying the causative organisms. It is important to recover and identify the pathogens through culture and biochemical tests.
The Widal price is ₹ 290. This cost includes the fastest home sample collection in 60 minutes of test confirmation, and the reports are available in 6 hours.
The Widal test, a blood test for typhoid, is used to identify antibodies in the blood that target specific antigens of Salmonella bacteria, notably Salmonella Typhi and Salmonella Paratyphi . It assists in diagnosing enteric fever, such as typhoid and paratyphoid fever, by revealing the body's immune response to these bacterial infections.
No, fasting is not necessary for the Widal test or Typhoid test at home.
Yes, Orange Health Labs allows you to book home sample collection for the Widal Blood test or Typhoid Blood test, ensuring sample collection within 60 minutes of booking, depending on slot availability.
Follow the below mentioned steps to book a blood test or health checkup on our platform:
Search for the Test : Search for the Widal test in Hyderabad or the Typhoid test in Hyderabad and click on Orange Health’s website.
Review and Book : Choose the test, review the test prerequisites, enter your address, and confirm the booking by picking a suitable time slot for sample collection.
Sample Collection : Our skilled eMedic will arrive within the selected time slot to collect the sample at your home.
Lab Processing : The sample will be transported to our NABL accredited and ICMR approved labs for analysis.
Receive Results : You will receive the reports of your Widal test at home via email or WhatsApp within 6 hours. They are also readily accessible on our app.
There is no standardised 'normal' range for the Widal test. Titres below 1:80 are typically considered negative, while titres of 1:80 or higher might suggest the presence of infection. However, the interpretation varies based on clinical factors and the prevalence of the disease in the specific region.
Once your sample is collected, you will receive your results within 6 hours through WhatsApp and the app if you reside in Hyderabad. Turnaround times may vary in other cities.
How often you undergo Widal testing is guided by your doctor's advice. If you have received a diagnosis of typhoid or paratyphoid fever, your doctor may recommend undergoing this test every 2 weeks, customised to fit your specific clinical status and symptoms.
The Widal test examines antibody titres against specific antigens of Salmonella bacteria, including the O and H antigens of Salmonella Typhi , along with the H antigens of Salmonella Paratyphi .
By detecting antibodies against Salmonella bacteria, the Widal test helps diagnose typhoid and paratyphoid fever.
The Slide method mixes serum and antigen on a slide, contrasting with the Tube method which mixes them in test tubes.
There is little risk associated with a blood test. You may feel slight discomfort at the needle puncture site during sample collection, but this discomfort is fleeting.
In the Widal test, a false-negative result inaccurately indicates the absence of antibodies when they are actually present, often due to testing too early. Conversely, a false-positive result incorrectly indicates the presence of antibodies, potentially influenced by previous infections or vaccinations.
Orange Health provides the quickest Widal test services in Hyderabad without the need to visit a physical lab. Localities include but are not limited to Banjara Hills, Jubilee Hills, Gachibowli, Madhapur, Hitech City, Kondapur, Begumpet, Somajiguda, Ameerpet, Panjagutta, Kukatpally, Miyapur, Manikonda, Secunderabad, Tarnaka, Abids, Koti, Mehdipatnam, Dilsukhnagar, Uppal, LB Nagar, Nallagandla, Nizampet, Attapur, Malkajgiri.
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Payment & Refund Policy
Test Name | Widal Test (Slide Method) |
---|---|
Price | The Widal Test price is ₹ 290. |
Also Known As | Typhoid test, Enteric Fever test or Widal Agglutination test |
Purpose | To identify Typhoid and paratyphoid fever. |
Pre-test Information | Fasting is not required for a Widal Test. |
Report Delivery | Get reports within 6 hours. |
Frequency | As prescribed by your doctor. |
Home Testing | Yes, home blood sample collection in 60 minutes from booking. |
Sample Required | A blood sample is required for a Widal test |
The Widal test cost in Hyderabad is ₹ 290. This Widal test price includes the fastest home sample collection within 60 minutes of booking, and the reports are available in 6 hours.