The Dengue NS1 Antigen ELISA test detects the non-structural protein 1 (NS1) of the dengue virus in blood. It targets specific antigens, which are foreign substances entering the body. This detection is possible in the acute phase, or within the initial 7 days of dengue fever, aiding in early diagnosis and timely medical care.

Dengue fever is spread by mosquito bites and is caused by one of the four serotypes of the dengue virus from the Flavivirus genus. When an infected mosquito bites a person, the virus enters the bloodstream and begins replication. This leads to the symptoms of dengue fever which are caused by the virus and the immune system’s response. The Dengue NS1 test can identify the virus in blood within the first 7 days of symptoms. Studies reveal that NS1 antigen levels in the blood correlate with the severity of the disease, with higher levels present in more severe infections. Hence, the Dengue Antigen test provides essential diagnostic information for dengue infection.

For Hyderabad residents experiencing dengue symptoms, a Dengue Antigen test is highly beneficial. Arrange your Dengue Antigen test in Hyderabad today if you suspect dengue exposure to confirm your status.

Here is how to prepare for a Dengue Fever NS1 Antigen test:

Prerequisites

Fasting is not needed for the Dengue NS1 test.

Best Time to Get Tested

The Dengue Antigen test can be conducted at any time during the day.

Who Should Consider a Dengue NS1 ELISA Test

You should consider this test if you have recently been in a dengue-endemic area and are experiencing symptoms such as high fever, rash, pain behind the eyes, nausea, vomiting, or muscle and joint pain.

Cautions Before Taking Dengue NS1 ELISA Test

No special precautions are required before taking the Dengue NS1 test.

It is advisable to take a Dengue NS1 test if you live in or have visited a region where dengue fever is prevalent and are currently experiencing symptoms of the illness. Dengue fever symptoms typically emerge within 4 to 10 days following a mosquito bite and persist for 3 to 7 days. These symptoms often include a rapid onset of fever up to 104°F or more, along with any of the following:

• Rash

• Headache

• Nausea and vomiting

• Severe pain behind the eyes

• Muscle, bone, or joint pain

When neglected, some individuals might develop dengue haemorrhagic fever, a life-threatening condition that causes abdominal pain, frequent vomiting, nosebleeds, bleeding gums, vomiting blood, blood in stools, and extreme tiredness. This underscores the need for early detection and treatment of dengue.

The NS1 antigen is present in the blood during the acute phase of infection, making the NS1 test effective within the first 7 days of symptoms. Beyond the first week, the test’s sensitivity decreases.

The Dengue Antigen test thus helps doctors detect dengue early, confirm the infection, and differentiate it from other similar diseases.

The table given below explains the interpretation of the results of a Dengue NS1 ELISA test:

Interference may occur in some cases where the blood sample contains antibodies against test components, such as during dengue virus testing, where cross-reactivity with other Flaviviruses or non-infectious conditions might result in false-positive results or misinterpretation. Thus, it's essential to consider the patient's clinical history for accurate diagnosis. Using both NS1 detection and the IgM Antibody test can enhance diagnostic accuracy for dengue infection. The dengue NS1 ELISA is preferred within the initial 7 days of symptom onset. After 7-10 days, IgG and IgM antibody tests are advised.